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You are here:Home » Molecular Biology » MB-Binding Proteins » COL1A1, Recombinant, Human (Collagen alpha-1(I) Chain, Alpha-1 Type I Collagen)

COL1A1, Recombinant, Human (Collagen alpha-1(I) Chain, Alpha-1 Type I Collagen)

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Specifications

Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha1(I) chains and one alpha2(I) chain, although homotrimers consisting of three identical alpha1 (I) chains have also been described. This recombinant mini pro-alpha1(I) collagen is a homotrimer of three shortened alpha1(I) chains with following domain structure from N-to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I(alpha1) and the C-terminal propeptide. This truncated pro-alpha1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
Catalog #C7510-11V
SourceChinese Hamster Ovary cell line, CHO-derived Gln23-Lys277+Gly1094-Leu1464, with an N-terminal human CD33 signal sequence and 6-His tag.
SDS-PAGE79-83kD, 54-56kD, 35-36kD, reducing conditions
ActivityMeasured by the cleavage of its C-terminal propeptide by recombinant human BMP-1. 50% of full-length Pro Collagen I alpha1 is cleaved by rhBMP-1.
Endotoxin Level 1EU/1ug (LAL)
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight65kD (full-length), 40kD, 28kD
SourceChinese Hamster Ovary cell line, CHO-derived
Purity~80% (SDS-PAGE).
FormSupplied as a liquid in Tris, sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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