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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I
collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.
Catalog #C7510-12
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA (Standard): 1:5000-1:50,000. Coat with 1ug Collagen Type I per well using A0030 as a substrate for 30 minutes at RT.
Western Blot (Non-reducing): 1:5000-1:50,000
Immunohistochemistry (Frozen): 1:50-1:200
Optimal dilutions to be determined by the researcher.
Note Some class specific anti-collagens may be specific for three dimensional epitopes which may result in diminished reactivity with denatured collagen on formalin-fixed, paraffin embedded tissues.
Recommended Secondary AntibodiesI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide.
PurityPurified by Immunoaffinity chromatography.
ImmunogenCollagen Type I from human and bovine placenta.
SpecificityRecognizes most mammalian Type I Collagens. Minimum crossreactivity to Type II, III, IV, V and VI Collagens. <1% crossreactivity with other types of collagen (ELISA). Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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