| Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I |
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| collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions. |
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| Catalog # | C7510-12 |
| Applications | Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA (Standard): 1:5000-1:50,000. Coat with 1ug Collagen Type I per well using A0030 as a substrate for 30 minutes at RT. |
| Western Blot (Non-reducing): 1:5000-1:50,000 |
| Immunohistochemistry (Frozen): 1:50-1:200 |
| Optimal dilutions to be determined by the researcher. |
| Note | Some class specific anti-collagens may be specific for three dimensional epitopes which may result in diminished reactivity with denatured collagen on formalin-fixed, paraffin embedded tissues. |
| Recommended Secondary Antibodies | I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide. |
| Purity | Purified by Immunoaffinity chromatography. |
| Immunogen | Collagen Type I from human and bovine placenta. |
| Specificity | Recognizes most mammalian Type I Collagens. Minimum crossreactivity to Type II, III, IV, V and VI Collagens. <1% crossreactivity with other types of collagen (ELISA). Non-specific crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
