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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E IH IC Gel

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #	C7510-12A
Applications	Suitable for use in ELISA, Dot and Slot Immunoblot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Dot and Slot Blot: Non-denaturing
	Immunohistochemistry (Frozen): 1:10-1:20
	Immunocytochemistry: 1:10-1:20
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 100mM borate buffered saline, pH 8.2.
Purity	Purified by af nity chromatography.
Immunogen	Human Type I collagen
Specificity	Recognizes conformational determinants on human type I collagen as demonstrated by ELISA. Exhibits <10% crossreactivity with collagen types II, III, IV, V and VI. Has not been tested for crossreactivity with other extracellular matrix proteins (e.g. laminin, bronectin). Species Crossreactivity: May crossreact with type I collagen from other species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.