| Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. |
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| Catalog # | C7510-13 |
| Applications | Suitable for use in RIA, Immunofluorescence, ELISA and Immunohistochemistry. Not suitable for Western Blot. Other applications not tested. |
| Recommended Dilution | RIA (Titer): 1:200 |
| Immunofluorescence (frozen mouse liver): 1:80 |
| ELISA: 1:200 |
| Immunohistochemistry (fixed, paraffin-embedded mouse tissues): 1:500 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | Not determined |
| Form | Supplied as a lyophilized powder. Reconstitute with 500ul sterile ddH2O. |
| Purity | Purified by column chromatography. |
| Immunogen | Collagen Type I from mouse skin |
| Specificity | Recognizes mouse Collagen Type I: 100%. |
| Crossreactivity | |
| Mouse Collagen Type III | < 1% |
| Mouse Collagen Type II, IV | < 0.1% |
| Human, chicken, rat Collagen Type I | < 0.1%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
