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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E IH IF RIA

Collagenases are endopeptidases that digest native collagen in the triple helix region. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity Unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase is unique because it can degrade both water-insoluble native collagens and water-soluble denatured ones. It can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions.
Catalog #	C7510-13B
Applications	Suitable for use in RIA, Immunofluorescence, Immunohistochemistry and ELISA. Also suitable for immunostaining extra or intracellular components in light microscopy and for the quantitation of Collagen Type I in biological uids or in culture supernatants. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:80 on frozen chicken tissues. Use with anti-rabbit IgG (FITC) conjugate.
	Immunohistochemistry (Immunoperoxidase technique): 1:1000 on fixed paraffin embedded chicken tissues.
	ELISA: 1:200
	RIA (Titer): 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Chicken
Concentration	Not determined
Form	Supplied as a liquid. No preservatives added.
Purity	Purified by Ion Exchange chromatography (DEAE-Trisacryl).
Immunogen	Chicken Collagen Type I extracted and purified from chicken skin.
Specificity	Recognizes chicken Collagen Type I
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.