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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E IF

Catalog #	C7510-13C
Applications	Suitable for use in IFA, SPRIA, and ELISA. Other applications have not been tested.
Recommended Dilution	IFA (indirect): 1:40 on frozen salmon tissues (skin, liver). Suitable for immunostaining of extra- or intracellular components in light microscopy in conjunction with anti-rabbit IgG-fluorescein conjugate.
	ELISA: 1:10-1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Salmon
Concentration	Not determined
Form	Supplied as a lyophilized powder. No preservative added. Reconstitute with 0.5ml sterile ddH2O or 40-50% glycerol.
Purity	Purified by ion exchange chromatography.
Immunogen	Collagen Type I extracted and puri ed from sh skin.
Specificity	Recognizes salmon Collagen Type I. Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.