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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E IF
Catalog #C7510-13C
ApplicationsSuitable for use in IFA, SPRIA, and ELISA. Other applications have not been tested.
Recommended DilutionIFA (indirect): 1:40 on frozen salmon tissues (skin, liver). Suitable for immunostaining of extra- or intracellular components in light microscopy in conjunction with anti-rabbit IgG-fluorescein conjugate.
ELISA: 1:10-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceSalmon
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with 0.5ml sterile ddH2O or 40-50% glycerol.
PurityPurified by ion exchange chromatography.
ImmunogenCollagen Type I extracted and puri ed from sh skin.
SpecificityRecognizes salmon Collagen Type I. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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