| Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I |
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| collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions. |
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| Catalog # | C7510-14C |
| Applications | Suitable for use in RIA, Immunofluorescence, Immunohistochemistry and ELISA. Other applications not tested. |
| Recommended Dilution | RIA Titer: 1:500 |
| Indirect Immunofluorescence (frozen human liver, skin, placenta): 1:80 |
| Immunohistochemistry: 1:500 Fixed, paraffin-embedded human tissues (skin, liver, placenta). |
| ELISA: 1:200 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies (IF) | I1904-40D: IgG, H&L (FITC) Pab Gt x Rb |
| I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-41B: IgG, F(ab')2 (FITC) Pab Gt x Rb |
| I1904-41G: IgG, F(ab')2, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-46N: IgG, H&L (FITC) Pab Gt x Rb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid. No preservative added. |
| Purity | Puri ed by ion exchange chromatography (DEAE-Trisacryl). |
| Immunogen | Collagen type I extracted and puri ed from human skin. |
| Specificity | Recognizes human Collagen Type I. Crossreactivity (RIA%) |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
