| Catalog # | C7510-14C1 |
| Applications | Suitable for use in RIA, Immunofluorescence, Immunohistochemistry and ELISA. Other applications not tested. |
| Recommended Dilution | ELISA: 1:100 |
| Immunofluorescence: 1:40 |
| Immunohistochemistry (Paraffin): 1:250 |
| RIA: 1:250 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies (IF) | I1904-40D: IgG, H&L (FITC) Pab Gt x Rb |
| I1904-40M: IgG, Fc, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-41B: IgG, F(ab')2 (FITC) Pab Gt x Rb |
| I1904-41G: IgG, F(ab')2, X-Adsorbed (FITC) Pab Gt x Rb |
| I1904-46N: IgG, H&L (FITC) Pab Gt x Rb |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2 before the addition of glycerol to 40%. No preservative added. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Collagen type I extracted and puri ed from human skin. |
| Specificity | Recognizes Collagen Type I from human and mouse samples. Crossreactivity with all other human collagens is negligible. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |