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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I, Digested (Collagen Type 1, Alpha-1 Type I Collagen, Collagen alpha-1(I) Chain, Collagen Type I alpha 1, COL1A1, COLIA1, Alpha-2 Type I Collagen, Collagen alpha-2(I) Chain, Collagen Type I alpha 2, COL1A2, Collagen Of Skin Tendon And Bone,

Anti -Collagen Type I, Digested (Collagen Type 1, Alpha-1 Type I Collagen, Collagen
alpha-1(I) Chain, Collagen Type I alpha 1, COL1A1, COLIA1, Alpha-2 Type I Collagen,
Collagen alpha-2(I) Chain, Collagen Type I alpha 2, COL1A2, Collagen Of Skin Tendon And
Bone,

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Collagens play a dominant role in maintaining the structural integrity of organs and tissues (1), but they also regulate cell polarity, migration, survival and phenotype, and provide diffusible signaling molecules following breakdown (2). Within the family of collagens that currently comprises 21 members, type I collagen is included in the group of fibril-forming collagens based on its structural and functional features. Type I collagen is ubiquitous in all vertebrates and is among the largest and most complex of all macromolecules. It accounts for 80-90% of all collagenous proteins in skin (3). The helical trimeric molecules of type I collagen compromise two alpha1(I) chains and one alpha2(I) chain, encoded by two separate genes Col(I)a1 and Col(I)a2, respectively (3). Matrix metalloproteinase (MMP)-1,-8, -13 and -14 cleave the peptide
bonds between residues Gly775 and Ile776 of the alpha1(I) chain and Gly775 and Leu776 of the alpha2(I) chains, resulting in ¼ and ¾ fragments.
Catalog #C7510-16D
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: ~2 times more reactive towards digested than intact type I collagen.
Western Blot (Non-reducing conditions): Reacts with both intact and digested collagen type 1.
Immunohistochemistry (Frozen): Preferentially recognizes collagenase-digested human type I collagen. Some cross reactivity with human epidermal keratinocytes. No cross reactivity with mouse collagen.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgM
Clone No2G8
HostMouse
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in 100mM Tris-HCl, pH 8, 0.05% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman type I Collagen, digested with Collagenase from Clostridium histolyticum
SpecificityRecognizes human Collagen Type I, Digested. Does not cross react with human type IV collagen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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