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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E IH IF

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #	C7510-17B
Applications	Suitable for use in Immunofluorescence, ELISA, RIA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: >1:80 on frozen rat tissues
	Immunohistochemistry (paraffin): >1:500
	ELISA: >1:200
	RIA (rat): 1: 200
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not Determined
Form	Supplied as a lyophilized powder. No preservative added.
Reconstitute with 500ul sterile ddH2O.
Purity	Purified by ion exchange chromatography (DEAE-Trisacryl).
Immunogen	Collagen Type I puri ed from rat skin.
Specificity	Recognizes rat collagen Type I. Does not crossreact with rat collagen Type III and rat elastin. Does not crossreact with collagen Type I from human and chicken sources. Crossreacts with mouse collagen Type I when tested by ELISA and Immunohistochemistry but does not crossreact when tested by RIA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.