| Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I |
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| collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions. |
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| Catalog # | C7510-17K |
| Applications | Suitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry. Not recommended for paraffin tissue sections. Other applications not tested. |
| Recommended Dilution | Dot and Slot Blot: 1:100-1:500 |
| ELISA: 1:50-1:100 |
| Immunohistochemistry (Indirect, frozen sections only): 1:10-1:20 |
| Immunocytochemistry (Indirect): 1:10-1:20 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added. |
| Purity | Purified by immunoaffinity chromatography. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose 4B. |
| Immunogen | Human placental collagen type I isolated by limited pepsin digestion and selective salt precipitation. |
| Specificity | Recognizes human type I collagen as demonstrated by ELISA. Less than 10% crossreactivity with collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
