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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Type I collagen is the most common of the collagens in vertebrates. It comprises up to 90% of the skeletons of the mammals and is also widespread all over the body. Such as: skin, tendons, ligaments, cornea, intervertebral disks, dentine, arteries and granulation tissues as the main locations. The importance of type I collagen for medical research is that it is involved in many human and animal diseases, including fibrosis, osteoporosis, cancer, atherosclerosis etc. Type I
collagen is frequently utilized to monitor physiological changes in tissues as well as being used as diagnostic tools in various pathological conditions.
Catalog #C7510-17K
ApplicationsSuitable for use in Dot and Slot Blot, ELISA, Indirect Immunohistochemistry and Indirect Immunocytochemistry. Not recommended for paraffin tissue sections. Other applications not tested.
Recommended DilutionDot and Slot Blot: 1:100-1:500
ELISA: 1:50-1:100
Immunohistochemistry (Indirect, frozen sections only): 1:10-1:20
Immunocytochemistry (Indirect): 1:10-1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives added.
PurityPurified by immunoaffinity chromatography. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose 4B.
ImmunogenHuman placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
SpecificityRecognizes human type I collagen as demonstrated by ELISA. Less than 10% crossreactivity with collagen types II, III, IV, V and VI. May show reactivity to type I collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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