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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IF
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #C7510-17S
ApplicationsSuitable for use in ELISA, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA: 1:4000
Indirect Immunofluorescence: 1:10-1:40 on frozen sections or cutured cells. Paraffin not recommended.
Western Blot: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS pH 7.5, 0.1% Mannitol, 0.1% Dextran. No preservatives added.
PurityPurified by ammonium sulfate precipation and Ion exchange chromatography.
ImmunogenHuman placental collagen type I
SpecificityRecognizes human collagen type I. Species Crossreactivity:
Rhesus monkey. Reacts with human collagen type II<2%, human collagen type III
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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