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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E B IF

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #	C7510-17S
Applications	Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilutions
	ELISA: 1:4000
	Indirect Immunofluorescence: 1:10-1:40 on frozen sections or cutured cells. Paraffin not recommended.
	Western Blot: 1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS pH 7.5, 0.1% Mannitol, 0.1% Dextran. No preservatives added.
Purity	Purified by ammonium sulfate precipation and Ion exchange chromatography.
Immunogen	Human placental collagen type I
Specificity	Recognizes human collagen type I. Species Crossreactivity:
Rhesus monkey. Reacts with human collagen type II	<2%, human collagen type III
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.