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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I (Biotin)

Anti -Collagen Type I (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300kD molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #C7510-17V
ApplicationsSuitable for use in ELISA, Dot and Slot Blots, Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Western Blots. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:4000
Dot/Slot Blot: 1:500-1:1000
Western Blot: reduced only
Indirect Immunohistochemistry (Frozen sections only): 1:20-1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Biotin.
PurityPurified by affinity chromatography.
ImmunogenHuman placental collagen type I
SpecificityRecognizes human Collagen Type I. Exhibits <10% crossreactivity with collagen types II, III, IV, V and VI. May crossreact with Collagen Type I from other species. Not tested for crossreactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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