Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #C7510-18Z
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlCartilage in lung or fetus.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No3F134
HostMouse
SourceChicken
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C7510-18ZX.
PurityPurified by Protein A affinity chromatography.
ImmunogenA puri ed preparation of lathyritic type II collagen from embryonic chicken sternum. Epitope
SpecificityHighly specific to chicken type II collagen and lathyritic type II collagen. Does not crossreact with types I, III, IV, V, VI, IX, X, or XI. In Western Blot, recognizes TCB fragment of pepsin-digested type II collagen after digestion with mammalian collagenase. Species Crossreactivity: human, mouse, rat and bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links