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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II (BSA & Azide Free)

Anti -Collagen Type II (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC IF
Catalog #C7510-18ZX
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlCartilage in lung or fetus.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F134
HostMouse
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-18Z.
PurityPurified by Protein A affinity chromatography.
ImmunogenA puri ed preparation of lathyritic type II collagen from embryonic chicken sternum. Epitope
SpecificityHighly specific to type II collagen and lathyritic type II collagen. Does not crossreact with types I, III, IV, V, VI, IX, X, or XI. In Western Blot, recognizes TCB fragment of pepsin-digested type II collagen after digestion with mammalian collagenase. Species Crossreactivity: human, mouse, rat, bovine, chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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