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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IF

Catalog #	C7510-19
Applications	Suitable for use in Immunofluorescence and Western Blot. Not suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1-2ug/ml for 2 hours at RT.
	Optimal dilutions to be determined by the researcher.
Positive Control	Cartilage
Cellular Localization	Cartilage matrix
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	0.N.225
Host	Mouse
Source	Chicken
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide before the addition of 40% glycerol.
Also available without BSA and azide at 1mg/ml-see cat# C7510-19X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	A purified preparation of lathyritic type II collagen from embryonic chicken sternum.
Specificity	Recognizes Type II collagen. Species crossreactivity: Human, chicken and mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.