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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IF

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #	C7510-19A
Applications	Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemsitry (formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.
	Optimal dilutions to be determined by the researcher.
Positive Control	Cartilage in lung or fetus
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.226
Host	Mouse
Source	Chicken
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C7510-19AX.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified type II collagen obtained from the adult chicken sternum by limited pepsinization. Epitope
Specificity	Recognizes chicken Type II collagen. Species Crossreactivity: human, mouse and salamander.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.