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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II (BSA & Azide Free)

Anti -Collagen Type II (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
Catalog #C7510-19AX
ApplicationsSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 min at RT.
Note: Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCL, pH 2.0 for 15 min at RT or 10 min at 37°C.
Optimal dilutions to be determined by the researcher.
Positive ControlCartilage in lung or fetus
Cellular LocalizationCartilage matrix
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.226
HostMouse
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19AX.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified type II collagen obtained from the adult chicken sternum by limited pepsinization.
SpecificityRecognizes chicken Type II collagen. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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