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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E IH RIA

Catalog #	C7510-19K
Applications	Suitable for use in Immunohistochemistry, ELISA, and Radioimmunoassay. Other applications have not been tested.
Recommended Dilution	ELISA: 1:100-1:200
	Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen rat articular cartilage tissue. Also reactive on paraffin embedded rat cartilage tissue: 1:250 using an ABC detection system.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2. No preservatives added.
Purity	IgG fraction
Immunogen	Collagen Type II extracted and purified from rat fetal cartilage.
Specificity	Rat Type II Collagen. Less than 0.1% reactivity with rat Collagen Types I, III, V, rat Elastin and Fibronectin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.