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You are here:Home » Antibodies » Antibodies-Collagen » Anti -Collagen Type II

Anti -Collagen Type II


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Clone Host Grade Applications
Polyclonal Rabbit Purified E IH RIA
Catalog #C7510-19K
ApplicationsSuitable for use in Immunohistochemistry, ELISA, and Radioimmunoassay. Other applications have not been tested.
Recommended DilutionELISA: 1:100-1:200
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen rat articular cartilage tissue. Also reactive on paraffin embedded rat cartilage tissue: 1:250 using an ABC detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservatives added.
PurityIgG fraction
ImmunogenCollagen Type II extracted and purified from rat fetal cartilage.
SpecificityRat Type II Collagen. Less than 0.1% reactivity with rat Collagen Types I, III, V, rat Elastin and Fibronectin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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