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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH RIA
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #C7510-19L
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Radioimmunoassay. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:40 for immunofluorescent staining of fresh frozen mouse articular tissue. Acetone or methyl-carnoy fixation is also reactive.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenCollagen Type II extracted and purified from mouse fetal cartilage.
SpecificityRecognizes mouse Collagen Type II. Shows <0.1% crossreactivity with mouse Collagen Types I, III and IV by radioimmunoassay.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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