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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Catalog #C7510-19M
Applications In Western blotting, antibody reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. Immunoblotting of CNBr peptides of collagen II shows that this antibody reacts with CB11 fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule. ELISA.
Recommended Dilution Western Blotting: 1:200 1:400 Cartilage is an appropriate positive control. Immunohistochemistry (Formalin/Paraffin): 12ug/ml. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris HCl, pH 2 for 15 minutes at RT or 15 minutes at 37°C.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q649
HostMouse
Concentration~200ug/ml
FormLiquid containing 10mM PBS, pH 7.4, 0.2% BSA and 15mM sodium azide.
PurityPurified immunoglobulin
ImmunogenPurified type II collagen; obtained from adult chicken sternum by limited pepsinization.
SpecificityRecognizes both alpha 1(II) and alpha 3(XI) chains which have identical primary structure. It also reacts with pepsin-digested type II collagen. Its epitope is localized in the triple helix of type II collagen. It shows no crossreaction with type I or type III collagen. Species Crossreactivity: Human, Chicken, Mouse, Salamander.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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