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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IF
Catalog #C7510-19N
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence (Indirect): 1:2-1:5 with fresh frozen, or acetone-fixed samples. Mild pepsin digestion is recommended.
ELISA: 1:100-1:500 with pepsin treated samples
Western Blot: 1:500-1:1000 with chemiluminescent detection.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q650
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. No preservatives added.
PurityAscites fluid
ImmunogenHuman (cartilage-specific) CNBr-cleaved Collagen II
SpecificityRecognizes both pepsin-solubilized and CNBr-cleaved human and bovine Collagen type II. No crossreactivity with Collagen types I, III, V and IX. Species Crossreactivity: Bovine, canine, human, mouse, porcine, rat, sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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