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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Catalog #C7510-19R
Applications Enzyme-Linked-Immunosorbent-Assay (ELISA)
Immunocytochemistry
Dot- and slot-immunoblotting
Immunohistochemistry (frozen sections)
Recommended DilutionImmunocytochemistry/Immunohistochemistry: 1:10-1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceBovine
Concentration~0.4mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified by affinity chromatography on bovine type II collagen covalently linked to agarose.
ImmunogenBovine type II collagen
SpecificityReacts with conformational determinants on bovine type II collagen as demonstrated by ELISA. May react with type II collagen from other species. Exhibits < 10% crossreactivity with collagen type I, III, IV, V and VI. Has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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