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Anti -Collagen Type II (BSA & Azide Free)
Anti -Collagen Type II (BSA & Azide Free)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IF |
|
| Catalog # | C7510-19X | | Applications | Suitable for use in Immunofluorescence, ELISA and Western Blot. Not suitable for use in Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml for 2 hours at RT. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Cartilage | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 0.N.225 | | Host | Mouse | | Source | Chicken | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Purified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix. | | Specificity | Recognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. Species Crossreactivity: Human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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