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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II (BSA & Azide Free)

Anti -Collagen Type II (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IF
Catalog #C7510-19X
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Not suitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlCartilage
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.N.225
HostMouse
SourceChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified preparation of lathyritic Type II Collagen from embryonic chicken sternum. Cellular Localization: Catilage matrix.
SpecificityRecognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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