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Specifications

Clone Host Grade Applications Polyclonal Rabbit Highly Purified E IF RIA

Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Catalog #	C7510-20B
Applications	Suitable for use in RIA, Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution	Immunofluorescence (Indirect): 1:40 on frozen bovine articular cartilage with uorescent anti-rabbit IgG conjugate.
	RIA:1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Bovine
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O.
Purity	Purified by ion exchange chromatography (DEAE-Trisacryl).
Immunogen	Collagen Type II extracted and purified from Bovine cartilage
Specificity	Recognozes bovine Collagen Type II. Species Crossreactivity: Bovine Collagen Type IX, <0.1%; Bovine Collagen Type XI <3.0%
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.