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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II

Anti -Collagen Type II

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E IF RIA
Catalog #C7510-20E
Application(s) For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type II in biological uids or in culture supernatants. Suitable for use in IFA, RIA, ELISA. Other applications not tested.
Recommended DilutionIndirect IFA: 1:80 with uoresceine anti-rabbit IgG conjugate on frozen chicken tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceChicken
Concentration~1mg/ml
FormLyophilized. Reconstitute with 0.5ml ddH2O.
PurityIgG fraction was obtained by ion exchange chromatography (DEAE-Trisacryl).
ImmunogenCollagen Type II extracted and puri ed from chicken fetal cartilage.
SpecificityChicken Type II collagen 100% Chicken Type I collagen <0.1% Chicken Type X collagen <0.1%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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