| Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. |
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| Catalog # | C7510-20F |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot (Non-denaturing): 1:5000-:10,000 |
| Standard ELISA: 1:4000-1:8000 |
| Immunohistochemistry (Frozen): 1:50-1:200 |
| Optimal dilution to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.125M sodium borate, pH 8.0, 0.075M sodium chloride, 0.005M EDTA, 0.01% sodium azide. |
| Purity | Purified by immunoaf nity chromatography. |
| Immunogen | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
| Specificity | Recognizes most mammalian Type II collagens and has negligible crossreactivity with Type I, III, IV, V and VI collagens. Nonspeci c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
