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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E IC

Catalog #	C7510-20P
SOURCE	Pooled antisera from hyperimmunized goats
CROSS ADSORPTION	Prior to af nity isolation, the anti-type II antisera is adsorbed against collagen types I,III, IV,V and VI immobilized on Sepharose 4B.
APPLICATIONS	Dot-and slot-blotting, Indirect immunohistochemical staining (frozen sections only), 1:20- 1:40, Enzyme linked immunosorbent assay (ELISA), 1:1000–1:4000, Immunocytochemistry
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Bovine
Concentration	0.4mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. Biotin conjugated
Purity	Purified by immunoaffinity chromatography.
Immunogen	Bovine type II collagen puri ed from nasal cartilage by limited digestion and selective salt precipitation.
Specificity	Reacts with conformational determinants on bovine type II collagen as demonstrated by ELISA. May react with type II collagen from other species. Exhibits <10% crossreactivity with collagen types I, III, IV, V and VI. The antibody has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.