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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type II (Biotin)

Anti -Collagen Type II (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IC
Catalog #C7510-20P
SOURCE Pooled antisera from hyperimmunized goats
CROSS ADSORPTION Prior to af nity isolation, the anti-type II antisera is adsorbed against collagen types I,III, IV,V and VI immobilized on Sepharose 4B.
APPLICATIONS Dot-and slot-blotting, Indirect immunohistochemical staining (frozen sections only), 1:20- 1:40, Enzyme linked immunosorbent assay (ELISA), 1:10001:4000, Immunocytochemistry
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceBovine
Concentration0.4mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. Biotin conjugated
PurityPurified by immunoaffinity chromatography.
ImmunogenBovine type II collagen puri ed from nasal cartilage by limited digestion and selective salt precipitation.
SpecificityReacts with conformational determinants on bovine type II collagen as demonstrated by ELISA. May react with type II collagen from other species. Exhibits <10% crossreactivity with collagen types I, III, IV, V and VI. The antibody has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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