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You are here:Home » Antibodies » Antibodies-Collagen » Anti -Collagen Type II (Biotin)

Anti -Collagen Type II (Biotin)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #C7510-21E
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000- 1:10,000.
ELISA: 1:10,000- 1:50,000.
Immunohistochemistry (frozen): 1:200- 1:1000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~1mg/ml (by UV absorbance at 280nm)
FormSupplied as a liquid in ,0.125.M sodium borate, 0.075M sodium chloride, 10mg/ml BSA (IgG and Protease free), 0.005M EDTA 0.1% sodium azide. Biotin conjugated
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type II from adult human knee cartilage and bovine nasal cartilage.
SpecificityRecognizes human Collagen Type II. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri ed standards. Some class speci c anti-collagens may be speci c for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- xed, paraf n embedded tissues. Recognizes most mammalian Type II collagens and has negligible crossreactivity with Type I, III, IV, V and VI collagens. Non-speci c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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