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You are here:Home » Kits and Assays » Kits-ELISA (Anti-Antigen Immunoglobulin), BioAssay™ » Collagen (Bovine) Type II, Mouse Antibody (IgG) BioAssay™ ELISA Kit

Collagen (Bovine) Type II, Mouse Antibody (IgG) BioAssay™ ELISA Kit


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This ELISA kit is designed to assay type II collagen antibodies in mouse sera. The microtiter strips provided in the kit have been coated with bovine type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.
Catalog #C7510-34
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.
This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure).
Kit ComponentsC7510-34E1: Microtiter Strips 10x8 wells
C7510-34E2: Reference Microtiter Strips 4x8 wells
C7510-34E3. Standard Antibody 1x 20u Dissolve in 1.25ml C7510-34E6: Sample/Standard Buffer to 16units/ml
C7510-34E4: Pab (HRP) 2x1 vial Dissolve one vial in 10ml of C7510-34E7 Secondary Antibody Buffer
C7510-34E5: Blocking Buffer 1x12ml
C7510-34E6: Sample/Standard Buffer 1x60ml Use for diluting standards and sample specimens.
C7510-34E7: Secondary Antibody Buffer 1x20ml
C7510-34E8: OPD 2x1 vial Dissolve one tablet in 20ml of C7510-34E9: OPD Buffer
C7510-34E10: Wash Buffer 20X dilute in 1L ddH2O.
C7510-34E11: Stop Solution 1x10ml 2N Sulfuric acid
Storage and StabilityStore components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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