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You are here:Home » Kits and Assays » Kits for ELISA, BioAssay™ » Collagen (Chicken) Type II, Rat Antibody (IgG) BioAssay™ ELISA Kit

Collagen (Chicken) Type II, Rat Antibody (IgG) BioAssay™ ELISA Kit

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Specifications

This ELISA kit is designed to assay type II collagen antibodies in rat sera. The microtiter strips provided in the kit have been coated with chicken type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.
Catalog #C7510-34A
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.
This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure).
Kit ComponentsC7510-34A1 Standard Antibody, 1x20units. Supplied as lyophilized powder.
C7510-34A2 IgG (HRP), 2x50ul. Peroxidase-conjugated goat anti-rat IgG. Supplied as lyophilized powder.
C7510-34A3 Blocking Buffer, 1x12ml
C7510-34A4 Sample/Standard Dilution buffer, 1x50ml
C7510-34A5 IgG (HRP) Dilution Buffer, 1x20ml
C7510-34A6 Stop Solution, 1x10ml. 2N sulfuric acid.
C7510-34A7 Wash Buffer (20X), 1x50ml
C7510-34A8 OPD Dilution Buffer, 1x20ml
C7510-34A9 OPD, 2 tablets
C7510-34A10 Microtiter Strips, 10x8-well strips. Coated with chicken collagen type II.
C7510-34A11 Reference Standard Strips, 4x8-well strips. Use 2 strips per run.
Storage and StabilityStore all components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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