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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type III

Anti -Collagen Type III

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Catalog #C7510-39X
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionELISA: 1:5000-1:50,000
Immunohistochemistry: 1:50-1:200
Immunohistochemistry(Paraffin): 1:20-1:200
Immunoprecipitation: 1:100
Western Blot: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, 0.1% sodium azide, pH 8.0
PurityPurified by immunoaffinity chromatography
ImmunogenCollagen Type III from human and bovine placenta
SpecificityRecognizes huma Collagen Type III. Species Cross-reactivity: mouse, rat and most mammals. Typically less than 1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCOL3A1, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), EDS4A, Fetal collagen, Type III collagen


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