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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type III (Biotin)

Anti -Collagen Type III (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IH IC
Catalog #C7510-43F
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Dot and Slot Immunoblot. Other applications not tested.
Recommended DilutionELISA: 1:1000-1:4000
Immunocytochemistry: 1:20-1:40
mmunohistochemistry (Frozen): 1:20-1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.4mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Biotin.
PurityPurified by Immunoaf nity chromatography.
ImmunogenHuman type III collagen puri ed from placental villi by limited pepsin digestion and selective salt precipitation.
SpecificityRecognizes conformational determinants on human type III collagen as demonstrated by ELISA. May react with type III collagen from other species. Exhibits <10% crossreactivity with collagen types I, II, IV, V and VI. The antibody has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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