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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E IH IC

Catalog #	C7510-43F
Applications	Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Dot and Slot Immunoblot. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:4000
	Immunocytochemistry: 1:20-1:40
	mmunohistochemistry (Frozen): 1:20-1:40
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50%) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.4mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Labeled with Biotin.
Purity	Purified by Immunoaf nity chromatography.
Immunogen	Human type III collagen puri ed from placental villi by limited pepsin digestion and selective salt precipitation.
Specificity	Recognizes conformational determinants on human type III collagen as demonstrated by ELISA. May react with type III collagen from other species. Exhibits <10% crossreactivity with collagen types I, II, IV, V and VI. The antibody has not been tested for reactivity with other extracellular matrix proteins (e.g. laminin, bronectin).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.