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You are here:Home » Antibodies » Antibodies-Collagen » Anti -Collagen Type III

Anti -Collagen Type III


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human (3) and kangaroo (4) skin, and of new canine tissue associated with biomaterial implants (2,5). If fixation of tissue is required, acetone or ethanol is recommended.
Catalog #C7510-43H
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:250
Immunohistochemistry: Unfixed, acetone or ethanol frozen sections.
Optimal dilutions to be determined by the researcher.
HybridomaNS1/AG4.1 myeloma cells with spleen cells from mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6F205
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide. before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenAcid-digested pepsin soluble human type III collagen. Epitope: Located ~100nm from the N-terminal of the molecule.
SpecificityRecognizes human type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). Species Crossreactivity: canine, rat and kangaroo type III collagens by ELISA and Western Blot. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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