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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, MGC93704, Type III Collagen)

Anti -Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor,
Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, MGC93704, Type III
Collagen)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH IC
Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I. Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with aortic aneurysm abdominal (AAA).
Catalog #C7510-44N
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
Immunocytochemistry: Cultured rat cells
ELISA: Native rat collagen III
Dot Blot: Native rat collagen III
Optimal dilutions to be determined by the researcher.
IHC Positive ControlRat kidney, skin
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenRat Collagen type III.
SpecificityRecognizes rat Collagen III. Minimal crossreactions with other collagens were only found if 10-20 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEhlers-Danlos Syndrome Type IV Autosomal Dominant, EDS4A, Fetal Collagen, FLJ34534


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