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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IH
As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. In immunohistochemistry no crossreactivity with other collagens at optimal dilutions. In immunoblotting, a slight crossreactivity with collagen type V is observed.
Catalog #C7510-50C
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:50-1:100
Western Blot: 1:50-1:100
Immunohistochemistry (Frozen tissue and paraffin sections): 1:50-1:100. Proteolytic treatment required; enzyme: pepsin. Incubate 1 hour at RT or overnight at 4ºC.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlSkin and kidney
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PuritySerum
ImmunogenHuman placental type IV collagen.
SpecificityRecognizes human collagen type IV of basement membranes and shows a homogeneous staining pattern in all tissues.
Species CrossreactivityRat, porcine, canine, feline, fish and mouse. In Western Blot a slight crossreactivity is observed with collagen type V .
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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