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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IH IC
Catalog #C7510-50H
ApplicationsSuitable for use in Dot and Slot Blot, ELISA, Immunohistochemistry (frozen) and Immunocytochemistry. Not suitable for use in Western Blot and Immunohistchemistry (paraffin). Other applications have not been tested.
Recommended DilutionsDot and slot blot: 1:1001:500
ELISA: 1:10001:4000
Immunohistochemistry (Indirect): 1:101:40 (frozen sections only)
Immunocytochemistry: 1:101:20
Optimal dilutions to be determined by the researcher.
Note Harsh chemicals or procedures involving heat may denature collagen epitopes. We recommend avoiding such actions when using this antibody.
Positive Controls kidney, muscle, tendon, spleen tissues
Negative Controls Neurons/glia
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0.
PurityAffinity purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose 4B.
ImmunogenHuman and bovine placental collagen type IV
SpecificityReacts with conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: Bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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