Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E IH IC |
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| Catalog # | C7510-50H | | Applications | Suitable for use in Dot and Slot Blot, ELISA, Immunohistochemistry (frozen) and Immunocytochemistry. Not suitable for use in Western Blot and Immunohistchemistry (paraffin). Other applications have not been tested. | | Recommended Dilutions | Dot and slot blot: 1:100–1:500 | | ELISA: 1:1000–1:4000 | | Immunohistochemistry (Indirect): 1:10–1:40 (frozen sections only) | | Immunocytochemistry: 1:10–1:20 | | Optimal dilutions to be determined by the researcher. | | Note | Harsh chemicals or procedures involving heat may denature collagen epitopes. We recommend avoiding such actions when using this antibody. | | Positive Controls | kidney, muscle, tendon, spleen tissues | | Negative Controls | Neurons/glia | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 100mM borate buffered saline, pH 8.0. | | Purity | Affinity purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose 4B. | | Immunogen | Human and bovine placental collagen type IV | | Specificity | Reacts with conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: Bovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base |
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