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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Catalog #C7510-50Y
ApplicationsSuitable for use in Immunoblot, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoblot: 1:5000-1:10,000
ELISA: 1:4000-1:8000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type IV from human and bovine placenta.
SpecificityRecognizes most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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