Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP IH |
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| Catalog # | C7510-52E | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:4000-1:8000 | | Western Blot: 1:5000-1:10,000 | | Immunohistochemistry (Frozen sections): 1:50-1:200 | | Optimal dilutions to be determined by the researcher. | | Note | Some class-speci c anti-collagens may be speci c for non-denatuted three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- xed, paraf n embedded tissues. Greatly diminished reactivity and selectivity of class specific anti-collagen antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in BBS, pH 8.0, 0.005M EDTA, 0.01% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Collagen Type IV from human and bovine placenta | | Specificity | Recognizes human Collagen Type IV. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri ed standards. Reacts with most mammalian Type IV collagens and has negligible crossreactivity with Type I, II, III, V or VI collagens. Non-speci c crossreactivity with other human serum proteins or non-collagen extracellular matrix proteins is negligible. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base |
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