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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Anti -Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen
Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #C7510-52E
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4000-1:8000
Western Blot: 1:5000-1:10,000
Immunohistochemistry (Frozen sections): 1:50-1:200
Optimal dilutions to be determined by the researcher.
Note Some class-speci c anti-collagens may be speci c for non-denatuted three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- xed, paraf n embedded tissues. Greatly diminished reactivity and selectivity of class specific anti-collagen antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in BBS, pH 8.0, 0.005M EDTA, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type IV from human and bovine placenta
SpecificityRecognizes human Collagen Type IV. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri ed standards. Reacts with most mammalian Type IV collagens and has negligible crossreactivity with Type I, II, III, V or VI collagens. Non-speci c crossreactivity with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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