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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3

Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide,
Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IH IC Gel
Human plasma proteins do not interfere with collagen binding. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina.
Catalog #C7510-53M
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
Immunocytochemistry: Cultured human cells
ELISA: Native human collagen IV
Dot Blot: Native human collagen IV
Optimal dilutions to be determined by the researcher.
IHC Positive ControlHuman kidney, liver, skin, heart
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenHuman Collagen type IV
SpecificityRecognizes human Collagen IV. Minimal crossreactions with other collagens were only found if 20-50 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCollagen Of Basement Membrane Alpha 1 Chain, Collagen Of Basement Membrane Alpha 2 Chain, RP11-90L1.2


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