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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3

Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide,
Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
COL4A1 (collagen IV a1) is a 185kD member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170kD a2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642aa in length. It has an N-terminal 7S proregion aa28-172, a central collagenous domain that contains multiple Gly-based repeats aa173-1440, and a C-terminal domain that is proteolytically cleaved to generate a 25-28kD NC1 globular segment that has potent antiangiogenic activity aa1441-1669. Multiple splice forms exist. One shows a deletion of aa499-849, a second shows a seven aa substitution for aa513-1669, and a third shows a seven aa substitution for aa958-1669. Over aa1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Catalog #C7510-53N
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11n50
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa1441-1669 from human Collagen IV a1 expressed in NSO cells (02462).
SpecificityRecognizes human Collagen IV a1. No cross-reactivity with recombinant human Collagen I alpha 1, III alpha 1, XIII alpha 1v4, XXIII alpha 1, or recombinant mouse Collagen XXV alpha 1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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