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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
Catalog #	C7510-55R
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 5-10ug/ml; reducing conditions; detects ~160kD band
	Immunohistochemistry: 1-10ug/ml using PLP-fixed, paraffin-embedded tissue. PLP fixation is required; antigen retrieval is HIER; not recommended for traditional formalin fixation; acetone fixation can also be used.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM,k
Clone No	2Q641
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.1M PBS, pH 7.0, 2% BSA (Protease free), before the addition of glycerol to 40%.
Purity	Purified
Immunogen	Human Collagen Type V
Specificity	Recognizes human type V collagen (CL(V)) at ~160kD. Species Crossreactivity: rabbit and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.