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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH IC

Catalog #	C7510-55W
Applications	• Enzyme-Linked-Immunosorbent-Assay (ELISA)
	• Immunocytochemistry
	• Dot- and slot-immunoblotting
	• Immunohistochemistry (frozen sections)
Recommended Dilution	Immunocytochemistry/Immunohistochemistry: 1:20-1:40
	ELISA: 1:1000-1:4000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Goat
Source	Human
Concentration	~0.4mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Purity	Purified by affinity chromatography on bovine and human type V collagen covalently linked to agarose.
Immunogen	Human type V collagen
Specificity	Reacts with conformational determinants on human type V collagen as demonstrated by ELISA. May react with type V collagen from other species. Exhibits < 10% crossreactivity with collagen type I, II, III, IV and VI. Has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.