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You are here:Home » Antibodies » Antibodies-Collagen » Anti -Collagen Type V (Biotin)

Anti -Collagen Type V (Biotin)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Catalog #C7510-55W
Applications Enzyme-Linked-Immunosorbent-Assay (ELISA)
Dot- and slot-immunoblotting
Immunohistochemistry (frozen sections)
Recommended DilutionImmunocytochemistry/Immunohistochemistry: 1:20-1:40
ELISA: 1:1000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
PurityPurified by affinity chromatography on bovine and human type V collagen covalently linked to agarose.
ImmunogenHuman type V collagen
SpecificityReacts with conformational determinants on human type V collagen as demonstrated by ELISA. May react with type V collagen from other species. Exhibits < 10% crossreactivity with collagen type I, II, III, IV and VI. Has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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