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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type VI

Anti -Collagen Type VI

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
Catalog #C7510-60J
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 10ug/ml
Immunohistochemistry: 1-10ug/ml on acetone fixed paraffin-embedded tissues or frozen tissues. Not recommended for formalin fixed tissues. Epitope retrieval suggestions are treatment with 0.04% trypsin in 0.01M CaCl2, 0.05M Tris-HCl, pH 7.6, 37ºC for 10 min before the non specific peroxidase block.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q637
HostMouse
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 2% BSA (protease-free).
PurityPurified
ImmunogenPurified human Collagen Type VI
SpecificityRecognizes human Collagen Type VI. Does not react with denatured or reduced type VI collagen, although this antibody does recognize the triple helix consisting of alpha1 (VI), alpha 2 (VI) and alpha3 (gamma). The epitope has not been mapped and is not known to react with collagens I, II, III, IV, or V in native assays.
Species CrossreactivityRabbit. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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