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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type VI (Biotin)

Anti -Collagen Type VI (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Catalog #C7510-60N
Applications Enzyme-Linked-Immunosorbent-Assay (ELISA)
Immunocytochemistry
Dot- and slot-immunoblotting
Immunohistochemistry (frozen sections)
Recommended DilutionImmunocytochemistry/Immunohistochemistry: 1:20-1:40
ELISA: 1:1000-1:4000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.4mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
PurityPurified by affinity chromatography on human type VI collagen covalently linked to agarose.
ImmunogenHuman type VI collagen
SpecificityReacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10% crossreactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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