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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type VI (Biotin)

Anti -Collagen Type VI (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Catalog #C7510-60Q
Cellular Localization Extracellular matrix.
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr). Other applications not tested.
Recommended DilutionDirect ELISA: 1:4000-1:8000.
Western Blot: 1:5000-1:10,000.
Immunohistochemistry (Fr): 1:50-1:200.
Immunoprecipitation: 1:5000-1:10,000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
Form0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type VI from adult human and bovine tissues.
SpecificityNegligible crossreactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: Crossreacts with Human and bovine. Expected to crossreact with all Mammals due to sequence homology. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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