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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Catalog #	C7510-61G
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2500-1:10,000 using 1ug of Collagen VI in a standard sandwich ELISA,
	peroxidase-conjugated streptavidin, 30 min., RT.
	Immunohistochemistry: 1:50 to 1:200. Frozen tissue sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and store at -20°C. Do not freeze. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Collagen Type VI from human and bovine placenta
Specificity	Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri ed standards. Some class speci c anti-collagens may be speci c for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- xed, paraf n embedded tissues. Recognizes most mammalian Type VI collagens and has negligible crossreactivity with Type I, II, III, IV and V collagens. Non-speci c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.