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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Catalog #	C7510-61J
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:2000-1:4000. 1ug of Collagen VI was used in a standard sandwich ELISA with Goat anti-Rabbit IgG [H&L] (HRP conjugated) and (ABTS (2,2'-azino-bis- [3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
	Western Blot: 1:250-1:2500
	Immunoprecipitation: 1:50
	Immunohistochemistry (Frozen tissue sections): 1:25-1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Collagen Type VI from human and bovine placenta
Specificity	Reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.