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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type VI, X-adsorbed

Anti -Collagen Type VI, X-adsorbed

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catalog #C7510-61J
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:4000. 1ug of Collagen VI was used in a standard sandwich ELISA with Goat anti-Rabbit IgG [H&L] (HRP conjugated) and (ABTS (2,2'-azino-bis- [3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
Western Blot: 1:250-1:2500
Immunoprecipitation: 1:50
Immunohistochemistry (Frozen tissue sections): 1:25-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type VI from human and bovine placenta
SpecificityReacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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