| The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. |
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| Catalog # | C7510-61Z |
| Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:10,000-1:1:50,000 |
| Western Blot: 1:5000-1:10,000 |
| Immunohistochemistry (FFPE): 1.25ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with C7510-61Z, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (Immunoglobulin and Protease Free), 0.01% sodium azide. Labeled with Biotin. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Collagen Type VI from human and bovine placenta |
| Specificity | Recognizes human Collagen, Type VI, Alpha 1 (COL6A1). Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
