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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant E IH

Type VII collagen is a newly characterized collagen which appears to be the major component of the anchoring fibril.
Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
Catalog #	C7510-64B
Applications	Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen): 1:5-1:10
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-65V: IgG (FITC) Pab RbxMo
	I1904-66A IgG X-Adsorbed (FITC) Pab RbxMo
	I1904-65W: IgG (HRP) Pab RbxMo
	I1904-66C: IgG X-Adsorbed (HRP) Pab RbxMo
Hybridoma	Sp2 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2036
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid in 0.2M Tris/HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Purity	Supernatant
Immunogen	Crude extract of skin.
Specificity	Recognizes the carboxyterminus of type VII collagen. Species Crossreactivity: human. Does not react with rat, mouse or porcine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.